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Tissue Culture of Palms & Cycads

Collectorpalms

By Collectorpalms
in DISCUSSING PALM TREES WORLDWIDE

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Collectorpalms Grand Master

Collectorpalms

Posted
Posted

Howdy! I know this had been brought up before. But is it correct that Date palms have  successfully been tissue cultured?

Also, I was under the assumption that you had to use the meristem? Basically killing the palm.

Regardless, I am interested in working with a tissue culture company, if it’s possible. I have some things in mind. 
 

Also, what about cycads?

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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kinzyjr Grand Master

kinzyjr

Posted
Posted

@Saber samiei had a few posts on it.  It does look like they were using the meristem:

https://www.palmtalk.org/forum/index.php?/topic/60089-determinate-meristem-of-date-palm-in-tissue-culture-thorn/

https://www.palmtalk.org/forum/index.php?/topic/55718-tissue-cultured-date-palm/

https://www.palmtalk.org/forum/index.php?/topic/55770-tissue-cultured-date-palm-production-from-off-shoots-apex/

https://www.palmtalk.org/forum/index.php?/topic/55802-heart-of-date-palm-extracted-for-tissue-culture-research/

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Lakeland, FL

USDA Zone 1990: 9a  2012: 9b  2023: 10a | Sunset Zone: 26 | Record Low: 20F/-6.67C (Jan. 1985, Dec.1962) | Record Low USDA Zone: 9a

30-Year Avg. Low: 30F | 30-year Min: 24F

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awkonradi Mentor

awkonradi

Posted
Posted (edited)

Somatic embryogenesis via tissue culture has been reported for Phoenix, Elaeis, Cocos, Bactris, Euterpe, and likely others.   I believe there are thousands of thus cloned Phoenix and Elaeis in plantations at this time.  Yes, some protocols sacrifice a palm, but I think cuttings from inflorescences also can be used.

Edited by awkonradi
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Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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awkonradi Mentor

awkonradi

Posted
Posted (edited)

See the attached for somatic embryogenesis from female Phoenix inflorescence.  I have a long-standing interest in this subject.  I'm an organic chemist, not a cell culture expert, but obtaining the chemicals and culture media, and sterilizing things, should be doable in my basement.  I would love to partner with motivated persons who are good at growing plants!

2016_Zayed_AnnAgSci.pdf

Edited by awkonradi
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Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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awkonradi Mentor

awkonradi

Posted
Posted (edited)

I would love to attempt cloning Jubaea x Syagrus, from inflorescence cuttings.

Edited by awkonradi
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Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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Collectorpalms Grand Master

Collectorpalms

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My last bit, about Cycads might have been missed. I hadn’t looked into this yet. 

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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awkonradi Mentor

awkonradi

Posted
Posted

I don't know about cycads.  Are any cycads agriculturally important?  If not, then research may be thin.

Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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Collectorpalms Grand Master

Collectorpalms

Posted
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not that I am aware, but the nursery trade In the USA has been increasing plants types in recent years that are only ornamental. 

  • Upvote 1

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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Collectorpalms Grand Master

Collectorpalms

Posted
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Posted

Using inflorescence cuttings, that would be a nice thought. 

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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rprimbs Mentor

rprimbs

Posted
Posted

Years ago I was going to try cell culturing rare Encephalartos from the meristem. 

You could do it.  You would need a 'clean room' with a laminar flow hood.  It would just take time and experimentation.  And it probably wouldn't make you much money in the long run.  I don't think the demand would be high enough.

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awkonradi Mentor

awkonradi

Posted
Posted

I would love to clone JxS, and just give away the clones on PalmTalk.

Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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Collectorpalms Grand Master

Collectorpalms

Posted
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Posted (edited)
44 minutes ago, rprimbs said:

Years ago I was going to try cell culturing rare Encephalartos from the meristem. 

You could do it.  You would need a 'clean room' with a laminar flow hood.  It would just take time and experimentation.  And it probably wouldn't make you much money in the long run.  I don't think the demand would be high enough.

You would have to be able to market to the masses to make a significant income. But if you can find something that the growers want to sell to the masses, box-stores ( tired of Pygmy and Majesties) then you might be onto something.

Edited by Collectorpalms

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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Collectorpalms Grand Master

Collectorpalms

Posted
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With the right equipment and protocol does anyone think it can be done outside of a research laboratory. I am think a Tissue Culture lab might want to a) charge a fortune b) take your idea and make it available to anyone. 

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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awkonradi Mentor

awkonradi

Posted
Posted
8 hours ago, Collectorpalms said:

With the right equipment and protocol does anyone think it can be done outside of a research laboratory. I am think a Tissue Culture lab might want to a) charge a fortune b) take your idea and make it available to anyone. 

I think it's time for me to put up, or shut up, and post pictures of callus formation, and developing plantlets, on PalmTalk.

Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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Darold Petty Grand Master

Darold Petty

Posted
Posted
11 hours ago, awkonradi said:

I would love to clone JxS, and just give away the clones on PalmTalk.

Andrei;  Do you know Richard Smith from Atherton ?  He was self-employed doing date palm tissue culture for many years.  He attempted to tissue culture the mule palms at the Lakeside Palmetum from an inflorescence.   He was able to get cells to reproduce and increase in number, but not able to get the material to differentiate into "root and shoot".

Disclaimer...  I have had no contact with Richard for many years. 

San Francisco, California

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awkonradi Mentor

awkonradi

Posted
Posted
20 minutes ago, Darold Petty said:

Andrei;  Do you know Richard Smith from Atherton ?  He was self-employed doing date palm tissue culture for many years.  He attempted to tissue culture the mule palms at the Lakeside Palmetum from an inflorescence.   He was able to get cells to reproduce and increase in number, but not able to get the material to differentiate into "root and shoot".

Disclaimer...  I have had no contact with Richard for many years. 

Thanks!  Very useful info.  No, I do not know Richard.  I will search for him.   Much progress has been published during just the last five years, including for Cocos.  I think maybe Cocos methods could be adapted to more Cocoids.

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Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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Collectorpalms Grand Master

Collectorpalms

Posted
  • Author
Posted

This is the same response I got from two companies today. 

636C725A-B32A-48F9-89B2-C63A6123FD6D.jpeg

Current Texas Gardening Zone 9a, Mean (1999-2024): 22F Low/104F High. Yearly Precipitation 39.17 inches.

Extremes: Low Min 4F 2021, 13.8F 2024. High Max 112F 2011/2023, Precipitation Max 58 inches 2015, Lowest 19 Inches 2011.

Weather Station: https://www.wunderground.com/dashboard/pws/KTXCOLLE465

Ryan (Paleoclimatologist Since 4 billion Years ago, Meteorologist/Earth Scientist/Physicist Since 1995, Savy Horticulturist Since Birth.)

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GeneAZ Rising Star

GeneAZ

Posted
Posted

Regarding cycads, I understand that they have been cultured to the point of leaf differentiation, but they are not able to produce roots on all attempts to this point.

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amh Grand Master

amh

Posted
Posted

I think this would have to be a passion project or for a nonprofit.

I dont think that a commercial company could justify the time and resources needed to clone and grow a palm this way.

The reality is that you could do this in your garage on a small scale fairly cheap.

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awkonradi Mentor

awkonradi

Posted
Posted

Attached is a paper about plantlet generation from Cocos inflorescence. 

2019_Shareefa.pdf

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Andrei W. Konradi, Burlingame, California.  Vicarious appreciator of palms in other people's gardens and in habitat

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sonoranfans Grand Master

sonoranfans

Posted
Posted

Our company has a tissue culture lab, we found that some genetics do better than others in tissue culture, and that is within the same species(hemp).  We select genetics that are robust in tissue culture in particular and that often comes with weaknesses in real world(outside) culture..  Find the right genetics and it could be useful, but I would support the sterile crosses like JxS.   With say a fertile "cold hardy" coco, you could unintentionally promote a highly disease suceptible genetic that propagates its weakness through the domestic population.   And the results wouldn't be known for years until after the damage is done.  

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Formerly in Gilbert AZ, zone 9a/9b. Now in Palmetto, Florida Zone 9b/10a??

 

Tom Blank

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