Culture medium composition for hybrid cocconut micropropagation from inflorescence

[Saber samiei]

II- Coconut: 
Plant material consisted of adult palms (20-25 years) belonging to the Malayan 
Yellow Dwarf variety and the Malayan Yellow Dwarf x West African Tall hybrids. 
Basal media used at all stages described below comprised Eeuwens inacroeleiilents 
and niicroeleinents ( 1976), Morel and Wetiiiore vitamins, sucrose (40g. l-'}, activated 
charcoal ( 2-3g 1- ) and agar (7,5g I-'). 1 
Callogenesis was performed with two different types of explant, immature, leaf and 
inflorescences. Leaf explants were fragments of iiiiiiiature leaves, sampled froni the 
spike wit hout wounding the apex. Inflorescence explants were fragments of rachillae 
from young inflorescences with an internal spathe length of between 15 and 40 CM. 
They were cultured on basal niedia suppleiiiented with 2,4-D (30 to 80 iiig 1-l) in test 
tubes placed in the dark at 2'i0C. Eiiibiyogenesis was induced by increasing t.he level 
of 2,4-D. Somatic embryo maturation was obtained by gradually reducing the 2,4-D 
concentration. Shoot developnient took place in light, on horinone-free inediuin with 
activated charcoal. Rooting required an auxin treatment with NU.

 

Refrence and its download link:

ADVANCES IN OIL PALM AND COCONUT MICRQPROPAGATION

 

https://www.google.com/url?sa=t&source=web&rct=j&url=https://www.researchgate.net/profile/Jean-Luc_Verdeil/publication/32975645_Advances_in_oil_palm_and_coconut_micropropagation/links/5643bbb508ae54697fb382ea/Advances-in-oil-palm-and-coconut-micropropagation.pdf&ved=2ahUKEwib_eDRlp3ZAhUDwxQKHa9vBkwQFjADegQIDRAB&usg=AOvVaw0xg9bAAltSppXL3LkqVKWv